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Stress often causes bacteria to form biofilms. Stress can manifest as a physical barrier, ultraviolet light, or a toxic substance such as antibiotics. The formation of these biofilms takes from several hours to days, and they can be of different shapes, sizes, colors, and textures depending on the type of bacteria. Being in a biofilm state protects them from harmful substances in the environment - biofilms have a unique outer wall with different physical and chemical properties than their individual cells. They can coordinate metabolism, slow down their growth, and even form an impenetrable barrier of wrinkles and creases. This is one of the ways they achieve high antibiotic resistance. Researchers from the United Kingdom recently studied the transition of the Hay Bacillus bacterium from a free-moving swarm to a biofilm as a defense mechanism and published what they did to combat its antibiotic-resistant properties in eLife.
To determine if their test strain was behaving like the others, they first ran stress tests on them. They tested the bacteria's response to the physical barrier, ultraviolet light, and antibiotics. The addition of a physical barrier led to the transition of bacteria from one layer to a multilayer layer, followed by an increase in cell density and the formation of multilayer islands near the barrier. Later, wrinkles formed on the islands near the barrier in the place where they began to appear initially.
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When they applied ultraviolet light to the swarm, they again observed a drop in cell speed and an increase in density. And after the scientists added a large dose of the antibiotic kanamycin, the bacterial cells formed a biofilm. The researchers then developed a strategy to combat this bacterial biofilm. They added kanamycin to the environment of a new batch of swarming bacterial cells and watched as the biofilm began to form. They then re-injected the antibiotic at a much higher dose than the first, just before the biofilm formation was complete. As a result, the partially formed biofilm was destroyed and bacterial cells died. This shows that antibiotic-resistant bacteria lose their resistance to antibiotics when they undergo a phase transition, right before going into the biofilm, where they will become much more resistant. Thus, with the correct administration of antibiotics, the bacteria can be attacked in their most vulnerable state and destroyed. The researchers believe that similar transitions from swarm to biofilm occur in other bacterial species. Their research may pave the way for finding more effective ways to control clinically relevant bacteria. For example, Salmonella enterica, which spreads into the bloodstream and is transmitted through contaminated food. Or Pseudomonas aeruginosa with multiple drug resistance, which after surgery causes infections in the blood, lungs (pneumonia) and other parts of the body and spreads in hospitals.
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Home education pitfalls.
We lived in a provincial town. I was very surprised that before us in a neighboring school from the 8th grade, parents took their son to family training. My father taught me. The headmaster of that school proudly announced that the boy returned a year later: "It was hard." The boy pulled it out, passed it well, but didn't want to do it again.
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The first pitfall
What happened to the first boy in family training in our city is the first pitfall. We came across it in the 5th grade. This is the desire of teachers to interrogate (do not ask!) a non-standard student on all issues. No student answers on all topics in all subjects. Only those who are in family training.
I was acutely aware of the teachers ' desire to prove that the system they serve is better. So that others don't get hurt. We found a way out: another school.
Choose the form of training: external. They will impose fewer requirements.
The second pitfall
My mother-in-law worked as a teacher all her life. The students loved her. She took our idea of home education badly. We were lucky to live in different cities.
Once she arrived for 2 days and, as it turned out, did not have time to go to school! I wanted to talk to the class teacher, so that the girl was asked more strictly, so that she didn't want to study at home.
It was a failed betrayal. Sticks in the wheels can be inserted by anyone who does not feel sorry for the child. Such people were always on the way. But my own grandmother...
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My parents didn't agree with our decision either. However, the grandfather wrote to his granddaughter: "Don't see that your mother and I disagree. Listen to your mother, no one loves you more than her."
Detractors are the second pitfall. Inevitable. The worst thing is when it's relatives.
The third pitfall
Natasha finished 10th and 11th grade in one year. 3 times I wanted to drop out of school. Overwork. What held her back was the thought of those who would also study as an external student after her. She knew that she was paving the wa
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